The links between subculture and animation are not particularly obvious, but they do exist, mainly in the form of parodies in satirical animations such as Southpark and The Simpsons for comic effect.
There are also animated characters that may appeal to a subculture for their attitudes and personalities, for example Daria's acerbic sarcasm and disdain for shallow high schoolers may appeal to Goths. However, Daria herself is not part of a distinct subculture, and neither is her appeal limited to a single subculture.
Similarly, while Tim Burton's style of animation is ostensibly gothic, but enjoys a broad fanbase fro multiple cliques and subcultures. Furthermore, in these films it is not just a few characters, but the entire animated world which share this style. Therefore no one is an outsider; gothic is the norm. A possible exception is Vincent, in which the eponymous character feels isolated and different from his wholesome american family, preferring art, poetry and reanimation to playing outside. Yet Vincent doesn't meet any like minded individuals with whom he could form a subculture.
Perhaps to create an animation solely aimed at or featuring a particular subculture would be to limit its appeal. Or maybe as the divides between subcultures are no longer as defined as they once were, it would not be pertinent to target one group, as viewers can recognise and identify with something deeper than the fashions of their favourite animated characters.